Discovery

Introduction to Dendritic Cells: Discoveryhttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1001/thumbnail.jp

Introduction to Dendritic Cells

Introduction to Dendritic Cellshttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1003/thumbnail.jp

Dendritic Cell and Immune Tolerence

Introduction ot Dendritic Cells: Dendritic cell and immune tolerencehttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1000/thumbnail.jp

Maturation of Dendritic Cells

Introduction to Dendritic Cells: Maturatin of Dendritic Cellshttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1005/thumbnail.jp

Migration of Dendritic Cells

Introduction to Dendritic Cells: Migratin of Dendritic Cellshttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1006/thumbnail.jp

Life History and Features

Introduction to Dendritic Cells: Life History and Featureshttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1004/thumbnail.jp

Dendritic Cells Initiate the Immune Response

Introduction to Dendritic Cells: Dendritic Cells Initiate the Immune Responsehttps://digitalcommons.rockefeller.edu/introduction-dendritic-cells/1002/thumbnail.jp

From secretome analysis to immunology: chitosan induces major alterations in the activation of dendritic cells via a TLR4-dependent mechanism

Dendritic cells are known to be activated by a wide range of microbial products, leading to cytokine production and increased levels of membrane markers such as major histocompatibility complex class II molecules. Such activated dendritic cells possess the capacity to activate na\"ive T cells. In the present study we demonstrated that immature dendritic cells secrete both the YM1 lectin and lipocalin-2. By testing the ligands of these two proteins, chitosan and siderophores, respectively, we also demonstrated that chitosan, a degradation product of various fungal and protozoal cell walls, induces an activation of dendritic cells at the membrane level, as shown by the up-regulation of membrane proteins such as class II molecules, CD80 and CD86 via a TLR4-dependent mechanism, but is not able to induce cytokine production. This led to the production of activated dendritic cells unable to stimulate T cells. However, costimulation with other microbial products overcame this partial activation and restored the capacity of these activated dendritic cells to stimulate T cells. In addition, successive stimulation with chitosan and then by lipopolysaccharide induced a dose-dependent change in the cytokinic IL-12/IL-10 balance produced by the dendritic cells

Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment

Dendritic cells play a key role in activation of the immune system as potent antigen-presenting cells. This pivotal position, along with the ability to generate dendritic cells from monocytes and ready uptake of antigen, makes them an intriguing vehicle for immunotherapy for a variety of indications. Since the first reported trial using dendritic cells in 1995, they have been used in trials all over the world for a plethora of indications. Monocyte-derived dendritic cells are generated from whole blood or apheresis products by culturing enriched monocytes in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). A variety of methods can be used for enrichment of monocytes for generation of clinical-grade dendritic cells and are summarized herein